Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.11.08 Master Mix
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8.11.08 Master Mix
| Reagent | Volume/Amount |
| Colony Suspension | 7.5 μl (not added to master, though) |
| Taq | 0.75 μl |
| Primer BT2736 | 6.0 μl 1 μM Stock |
| Primer BT2737 | 6.0 μl 1 μM Stock |
| dNTPs | 1.5 μl 10 mM Stock |
| DMSO | 3.75 μl |
| Buffer | 7.5 μl |
| Mg2+ | 3.75 μl |
| dH20 | 38.25 μl |
| Total | 75 μl |
Aliquotted 9 μl of to each of 7 tubes, then added 1 μl appropriate colony suspension, prep, and genomic DNA template.
