Matt Gethers/CRI, Thailand/Labwork/Gels/Week of 6.29.08
6.29.08
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 |
| 5 μl λ DNA Ladder | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) 50oC | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) 65o |
100 volts, 30 minutes, 1% gel, 20 minutes staining. This was a gradient PCR to determine the optimum annealing temperature. All lanes showed product (50 to 65 degrees).
6.30.08
Gel #1
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 |
| 5 μl λ DNA Ladder | 5 μl 2736/2737 6.27.08 pKn001.1 transformation colony PCR Product #1 (Taq) | 5 μl 2736/2737 6.27.08 pKn001.1 transformation colony PCR Product #2 (Taq) | 5 μl 2736/2737 6.27.08 pKn001.1 transformation colony PCR Product #3 (Taq) | 5 μl 2736/2737 6.27.08 pKn001.1 transformation colony PCR Product #4 (Taq) | 5 μl 2736/2737 6.27.08 pKn001.1 transformation colony PCR Product #5 (Taq) | 5 μl 2736/2737 6.27.08 pKn001.1 transformation colony PCR Product #6 (Taq) | 5 μl 2736/2737 6.27.08 pKn001.1 transformation colony PCR Product #7 (Taq) | 5 μl 2736/2737 Positive Control (genomic DNA template, Taq) |
100 Volts, 30 minutes, 1% gel, stained for 20 minutes. The positive control worked beautifully and it looks like colony 3 turned up a product at the correct length.
Gel #2
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 |
| 5 μl λ DNA Ladder | 5 μl M13_for/BT2696 6.27.08 pKn002.2 transformation colony PCR Product #1 (Taq) | 5 μl M13_for/BT2696 6.27.08 pKn002.2 transformation colony PCR Product #2 (Taq) | 5 μl M13_for/BT2696 6.27.08 pKn002.2 transformation colony PCR Product #3 (Taq) | 5 μl M13_for/BT2696 6.27.08 pKn002.2 transformation colony PCR Product #4 (Taq) | 5 μl M13_for/BT2696 6.27.08 pKn002.2 transformation colony PCR Product #5 (Taq) | 5 μl M13_for/BT2696 6.27.08 pKn002.2 transformation colony PCR Product #6 (Taq) | 5 μl 2736/2737 6.27.08 pKn002.2 transformation colony PCR Product #7 (Taq) | 5 μl M13_for/BT2696 Positive Control (genomic DNA template, Taq) |
100 Volts, 30 minutes, 1% gel, stained for 20 minutes. Nothing showed up at all, including the positive control.
7.2.08
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 |
| 5 μl λ DNA Ladder | 5 μl M13_rev/BT1033 6.27.08 pKn002.2 transformation colony PCR Product #1 (Taq) | 5 μl M13_rev/BT1033 6.27.08 pKn002.2 transformation colony PCR Product #2 (Taq) | 5 μl M13_rev/BT1033 6.27.08 pKn002.2 transformation colony PCR Product #3 (Taq) | 5 μl M13_rev/BT1033 6.27.08 pKn002.2 transformation colony PCR Product #4 (Taq) | 5 μl M13_rev/BT1033 6.27.08 pKn002.2 transformation colony PCR Product #5 (Taq) | 5 μl M13_rev/BT1033 6.27.08 pKn002.2 transformation colony PCR Product #6 (Taq) | 5 μl M13_rev/BT1033 6.27.08 pKn002.2 transformation colony PCR Product #7 (Taq) | 5 μl M13_rev/BT1032 Positive Control (genomic DNA template, Taq) |
100 Volts, 30 minutes, 1% gel, stained for 20 minutes. Every lane had some product, but there were usually multiple bands (some at ~600, then 1500, and one higher, I think). Of all the colony PCR lanes, 1 and 2 had the brightest bands at ~600.
7.4.08
| Lane 1 | Lane 2 |
| 5 μl λ marker | 20 μl pKn002.P1 HindIII/SphI digest |
100 volts, 30 minutes, 1% agarose. Stained in EtBr for 15 minutes. Excised band at ~4.2 Kb.
