Matt Gethers/CRI, Thailand/Labwork/Digests/PstI/8.12.08 Gel Results
8.12.08 Diagnostic Digest Gel
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5||Lane 6||Lane 7||Lane 8||Lane 9||Lane 10||Lane 11|
|5 μl λ ladder||10 μl pIs001.P1 in water||10 μl pIs001.P1 digest||7.5 μl pIs001.P9a in water||7.5 μl pIs001.P9a digest||7.5 μl pIs001.P9b in water||7.5 μl pIs001.P9b digest||7.5 μl pIs001.P10a in water||7.5 μl pIs001.P10a digest||7.5 μl pIs001.P10b in water||7.5 μl pIs001.P10b digest|
120 Volts for 20 minutes, 1% gel. Stained in EtBr for 15 minutes. Definitely two bands from one looking at the P1 digest. Everything else in unaffected by the PstI and the bands run lower than P1 undigested. Looks like the ORF is gone despite the extra amp and glucose.