Map

Mapping strategies

Goals:

• to see in genR was inserted (length of DNA should be the length of pUC-ampR plasmid+ length of genR). Chose a restriction site found once in pUC-ampR plasmid to linearize it.
• If genR was inserted, for the zinc-fingered transposases, we want to see WHERE the cassette was inserted, so we can measure the specificity of insertion.
  • We'll have to research where the zinc-fingered transposases bind and cut the DNA