Manual 2ab Assembly Digest
From OpenWetWare
Jump to navigationJump to search
Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix.
Lefty MasterMix (for 4uL miniprep DNA)
- 4uL water
- 1uL of NEB2
- 1uL XhoI
- 1uL BamH1
Righty MasterMix (for 4uL miniprep DNA)
- 4uL water
- 1uL of NEB2
- 1uL XhoI
- 1uL BglII
Incubate for 1 hour at 37deg C.
