Kubke Lab:Research/CND/Records/HK029

EmbryoName HK029- chicken embryo- Stage early 20 - 12.1.15

First embryo that I am using for my data collection experiment

Experiment

Objective To dye seperate branches of each trigeminal nerve different colours to follow their cell bodies and see how they are organised. Also the dye each side of the same nerve a seperate colour so that I can notice any decussation or cross talk at the floorplate.

Procedure

• Trigeminal nerve L- Ant branch DiO and post branch DiI, opp on R side

Notes nerves were cleanly dissected I feel but was purposefully light on the injection because I want to see how much I need to strike the balance between bright dyes but also then clear images with lots of detail

Results

V

(green and orange)

Anterior Branch

• Afferents

• Efferents

• Other

Posterior Branch

• Afferents

• Efferents

• Other

Facial and VIII

(Green and Orange on opposite sides)

• Afferents

• Efferents

Summary

Questions

Future