Jonesma Lab:lba
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- Add 20g LB powder to 800 mL dH2O
- Mix to dissolve
- Add 3.2g Agar to a 500 mL bottle
- Add 400 mL dissolved LB to each bottle
- Autoclave
- Once autoclaved use microwave to melt (Careful, 5min on high power usually sufficient)
- Leave to cool (50°C waterbath) until comfortable to hold
- Add antibiotics of choice
- Pour plates and leave to set