Jhdavis::Flping
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- Transform pCP20 (p202 for me) - this is ampR and ts - grow at 30C
- grow colony at 30, then streak out on LB only plate, plate at 42
- pick colony, grow up and streak out on lb only, lb/amp, lb/markertrying to remove, plate at 30.
- save clone if it shows correct phenotype