Jessica Karen Wong/Notebook/2007-7-23
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- Minipreped I2055 for seq w/ VF and VR
- Got some sequencing redo's back and I2055-3K3 which didn't work, nothing incredibly enlightening
- Did a scarring/promoter insert PCR of I2056 on a gradient (50-56) w/ supermix
- Ran a gel, all lanes looked good
- Doing a 100ul PCR of I2056 at 51 overnight
- Wrote up proposal for synthesis of I2055
- Ordered new primers
- CTTAGTAG GCGGCCGC ACTAGT G TCACACAGGAAAGTACTAGATGCG, BBbp_E0240_Fwd_Spe
- CTTAGTAG GCGGCCGC TCTAGA G TCACACAGGAAAGTACTAGATGCG, BBbp_E0240_Fwd_Xba
- CTTAGTAG GCGGCCGC ACTAGT G TCACACAGGAAAGTACTAGATGGC, BBbp_I2057_Fwd_Spe
- CTTAGTAG GCGGCCGC TCTAGA G TCACACAGGAAAGTACTAGATGGC, BBbp_I2057_Fwd_Xba
- TCAGCGAT GCGGCCGC ACTAGT A GTGCTCAGTATCTCTATCACTGATAGG, BBbp_R_Rev_Spe
- CTTAGTAG GCGGCCGC TCTAG ATGCGTAAAGGAGAAGAACTTTTC, BBbp_I2055_Fwd_Xba
- CTTAGTAG GCGGCCGC TCTAG ATGGCTTCCTCCGAAGACG, BBbp_I2056_Fwd_Xba