Jessica Karen Wong/Notebook/2007-6-20
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Added LacZ to the registry
- Part Number BBa_I2054
- Got sequence from NCBI
- Original had an EcoR1 site at bp 3019
- Performed a point mutation at bp 3021 - changed A to G
- Both GAA and GAG are codons for gultamic acid
Ordered Primers for I2054
- I2054_F -- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATGACCATGATTACGGATTCACTG
- I2054_R -- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTATTATTTTTGACACCAGACCAACTGG
- Bold is the tail used to build a Biobrick listed in Synthetic Biology:BioBricks/Part fabrication
