July 6, 2010
- Autoclaved glassware
- Made Terrific Broth without antibiotics
- Grew out CFP, YFP, and GFP
- Did a transformation of RFP that has a tetracycline resistance (RFP biobrick into TOP10 cells)
- Made solutions of copper solutions and TOP10 cells to determine optimal growth environment
- Analyzed alignment utilizing BLAST of construct templates and primer sequences
- Transformed YFP biobrick into TOP10 cells
- Grew out YFP, CFP, and YFP from glycerol stock for testing of fluorescence using the plate reader in the morning
- Contacted team about next regional meeting
- Discussed skype calls and established set individuals on each team that would be in charge of maintaining communication over skype
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