IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/29

iGEM Project name 1

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June 29, 2010 Performed a mini prep on CFP and YFP- (1.5 ml vs. 3ml- 1.5 ml twice) AC-CFP-A 60.0 ng/ul DN-CFP-A 55.3 ng/ul AC-CFP-B 54.8 ng/ul DN-CFP-B 64.4 ng/ul AC-YFP-A 40.7 ng/ul DN-YFP-A 54.9 ng/ul AC-YFP-B 57.9 ng/ul DN-YFP-B 57.9 ng/ul Quantified DNA and analyzed results of the mini prep Analyzed the second PCR product Performed rapid transformation using 1st PCR product from june 28th for future stockpile Prepared plates from the two YFP glycerol stocks for comparison Prepared overnight culture of RFP Worked on regional meeting presentation