IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/23

iGEM Project name 1

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June 23, 2010 Attended seminar: Impact of Evolutionary Changes in Organisms or Malignant Tumor Cells, David Mittelman, Baylor College of Medicine Redesigned primers and sent primer order to Julie for review Looked up RBS sequences and compared their efficiencies Discussed ideas for optimization Received TOPO TA vector Graphed and analyzed fluorescence data Made LB broth Made overnight cultures of TPO10, CFP, RFP, and YFP cultures for another fluorescence reading