IGEM:VGEM/2007/Notebook/2007-6-8

• Making Glycerol Stock of GFP transformed cells

1. We verified the expression of GFP in our cells. It was a great moment in the dark Wilsdorf closet.
2. We made a 50:50 cell to glycerol solution and stored it in the minus 20C.
3. We plated another 200uL of cells onto Amp+ plates. We hope to have more colonies on this plate.

ERuhi 11:58, 8 June 2007 (EDT)

Protocol: Making glycerol stock for long term storage:

  1. Add 1 ml of 40% glycerol in H2O to a cryogenic vial.
  2. Add 1 ml sample from the culture of bacteria to be stored.
  3. Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
        1. Alternatively, pipet to mix.
  4. On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
  5. Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.

Note: We were sucessful in transforming our cells with the biobrick ERuhi 21:41, 8 June 2007 (EDT)