IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/10/26
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10.26.10Minipreps were made from:
The plasmids that only had the single part were put into the cryostock and labeled:
Digestions were ran for the following:
Sequencing of; golS + RBS + Terminator, golB + RBS + Terminator, golT + RBS + Terminator was done using the VF2 primer to verify. A gel for the earlier digestions was done and parts were extracted from the gel. Ligations using the extracted genes from the gel:
After the ligations, PCR was used to verify that the inserts were in the plasmid backbone using VF2/VR primers. The ligations were put into cells via heat shock transformation. | |
