IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/05/28

UIUC - Arsenic Collection System!

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5/28/10 Bootcamp Day 5 9:00 Set up plate for plate reader Our samples were in lanes 2 and 3, rows B-G, 250 ul of sample in each well. Row Lane Sample B 12M 1 12O1 C 12M1 12O1 D 12M1 12O1 E 12M2 12O2 F 12M2 12O2 G 12M2 12O2 9:10 Miniprep. 22 ul final volume, used 2 ul to nanodrop. Concentrations 12M 324 ng/ul 12O 353 ng/ul 10:00 PCR Ingredient amount/reaction Mastermix (16 reactions) 10 mM dTNPs .5 ul 8 ul 10X buffer 6 ul 96 ul Primer F .5 ul 8 ul Primer R .5 ul 8 ul DNA 2 ul added individually to each reaction H2O 20.3 ul 324.8 ul Polymerase .2 ul added individually to each reaction added 27.8 ul mastermix, .2 ul polymerase, and 2 ul DNA template to each reaction. Samples: 12M, 12O Primers: Biobrick Prefix, Biobrick Suffix Program: iGEM001