IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/04

iGEM Project name 1

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Floor One Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays. The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent E. coli cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction.