IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/08/30

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July 31, 2009

Growth Assays Added PNP to these cultures at approximately 0.2 OD (660). This is exponential growth phase.

3 ml cultures, so 

             1 mM = 30 uL PNP
             0.5 mM = 15 uL PNP
             0.1 mM = 3 uL PNP

4.1C 

          OD 12:30 = 0.06, 1:30 = 0.13, 2:30 = 0.093, 4:15 = 0.255

4.1 .1 

          OD 12:30 = 0.05, *1:30 = 0.16, 2:30 = 0.078, 4:15 = 0.085

4.1 .5 

          OD 12:30 = 0.06, 1:30 = 0.13, *2:30 = 0.147, 4:15 = 0.062

4.1 1.0 

          OD 12:30 = 0.06, *1:30 = 0.239, 2:30 = 0.082, 4:15 = 0.059

3 C 

          OD 12:30 = 0.151, 1:30 = 0.290, 2:30 = 0.443, 4:15 = 0.549

3 0.5 

          OD 12:30 = 0.126, *1:30 = 0.326, 2:30 = 0.226, 4:15 = 0.477

3 1.0 

          OD 12:30 = 0.076, *1:30 = 0.218, 2:30 = 0.109, 4:15 = 0.245

2 C 

          OD 12:30 = 0.085, 1:30 = 0.128, 2:30 = 0.169, 4:15 = 0.230

2 0.5 

          OD *12:30 = 0.291, 1:30 = 0.144, 2:30 = 0.172, 4:15 = 0.212

2 1.0 

          OD 12:30 = 0.070, *1:30 = 0.269, 2:30 = 0.177, 4:15 = 0.095

Notes: Point is both to check for 1. GFP expression and to 2. Assay the effects of PNP on cell growth. use of 3 different molarities on strain 4.1 will show relative concentration's effects on growth

  • = time of PNP inductions

End of Notes



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