IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/11

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May 11th, 2010 Modeling Luciferase We are working for Luciferase model. The units will be measured from SI, not RLU; this is to standardize inputs and outputs. The emission need be measured in vitro vs in vivo because there are differences for cytosolic Luciferin and free luciferin. In literature data shows enzyme saturation in mice for inoculations with luciferin: 1.55mM-> Cytosolic luciferin 134 μM-> Free luciferin. -For this we need to measure the relation between substrate-enzyme and luciferin in-out cytosolic. A [Luc-ina]cyt = [Luc-ina]RECY + K[Luc-ina]NON-CYT + K’[Luc-ina]cyt – R[Luc-ina]cyt – D[Luc-ina]cyt K = Constant of permeability of influx. K = Constant of permeability of efflux. R= Constant of activity. D= Constant of degradation. In literature the constant of activity for Luciferase = 4.83 e-6 mol/seg. For these constants we need: -Degradation rate -Reaction rate (Luciferase activity). -Rates of permeability. This equation will serve to maximize the activity of luciferase and light creation. Regenerating luciferin enzyme Other stuff Search for kits of Luciferin inoculation.