IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/25

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An error

Today the team realized that the standardization had an error, because a fragment neccesary was absent in our pBBR1MCS-5 standard plasmid. this is because when we didn't amplify the full standard region form the pSB1T3 plasmid (which was inserted into pBBR1MCS-5), due to an error designing the forward primer. I re-designed it and will repeat all the experiment, the new primer is:

>RFP-UP-Ok///melting60.8°C

CGG GCC CGA GTG CCA CCT GAC GTC TAA GAA ACC ATT ATT AT

This primer will be ready on monday



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