IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/07/20

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Many Isolation of standard pBBR1MCS-5 Our partner Gus needs a lot of plasmid backbone with sitcky ends done by EcoR1 and Pste1 in order to insert 22 constructions, carrying a RFP all of them. But we need to be careful because the plasmid already has RFP so I need to extract many plasmid, digest it and isolate it from band. Today I did 9 digestions and here are the results: Lanes: 1. Ladder 10 kb 2. Isolation 1 3. Isolation 2 4. Isolation 3 5. Isolation 4 6. Isolation 5 7. Isolation 6 8. Isolation 7 9. Isolation 8 10. Isolation 9 The only failed isolation is the eigth.