BioFilm Track
- Made 200ml PBS buffer solution for washing biofilm
- Leaving 96 well plates with staph strain RN4220 for 48hr incubation - Staph strain RN4220 most likely would not form biofilm according to literature references
Started "tube method" to test staph strain 8325 for biofilm formation
- Time placed in incubator; 4pm (20 hours incubation, 37C, static condition)
Content
- 10ml TSB in glass tube
- 5 % w/v glucuose (0.5g)
- 2 % w/v NaCl (To facilitate biofilm growth)
- Staph strain 8325 obtained from Lando
(Heated TSB to dissolve glucose, NaCl. Cooled before adding staph)
dspB Track
Gel Verify
Gel Verify PCR products from last day
Protocol: gel verification protocol in Protocol binder (SOP)
- Changes: Used 1% agarose gel instead of 0.8%
Gel orientation:
Gel orientation
| A | B | C | D | E | F | 100bp ladder | G | H | I | J | K | L | M | Machine conditions: 100V, 45 min, 0.5X TBE Buffer
Results:
Vicki Ma 02:40, 4 August 2010 (EDT)
Restriction Digest(ON)
Protocol: Restriction digest protocol in Protocol binder (SOP)
Table 4. Restriction Digest Supermix
| REAGENTS | 1 RXN VOLUME (uL) | SUPERMIX |
| Buffer 2 | 5 | x6 | 30 |
| BSA | 0.5 | x6 | 3 |
| ddH2O | 37 | x6 | 222 |
| EcoRI | 1 | x6 | 6 |
| PstI | 1 | x6 | 6 |
- DNA: Add 5uL of each A,B,C,D,E,F, psb1A3 (backbone plasmid) [a,b,c,d,e,f]
- Left ON in 4C fridge on ice
Vicki Ma 03:08, 3 August 2010 (EDT)
Misc
Poured 2* 400ml Amp plate + 1 * 400ml Chloro plate
|
iGEM Project name 1
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