Biofilm track
Jason G
- 2 streaked plates (from june 30th) taken out from 37C box at 8:35am
- Staph grew on both plates without any signs of contamination.
- Indicates that glycerol stock of staph made from original liquid culture (June 30th) can be used for OD testing.
- Staph on streaked plates can be used.
- Grew ON culture (aerated, 32.5C, 180 rpm) of staph from streaked plates and glycerol stocks: 2 for OD measurement,
2 for staph competent cell growth.
Time inserted: 4:00pm
To be taken out at 10:00am (10:00 am on July 02)
dspB Track
Marianne and Vicki
Gel verification of colony PCR products (from last day)
See protocol: Gel verification protocol in iGEM training manual
Gel box #1: Lagally Box: 32 wells --> 8T1-8T20, 11T1-11T9, two 100bp ladders, W1
Table 1. Gel box #1 arrangement
layer 1 | 8T1 | 8T2 | 8T3 | 8T4 | 8T5 | 8T6 | 8T7 | 8T8 | 8T9 | 8T10 | 100bp ladder | 8T11 | 8T12 | 8T13 | 8T14 | 8T15 | 8T16 | 8T17 | 8T18 | 8T19 |
layer 2 | 8T20 | 11T1 | 11T2 | 11T3 | 11T4 | 100bp ladder | 11T5 | 11T6 | 11T7 | 11T8 | 11T9 | W1 |
Gel box #2: Jefferies Lab Box: 20 wells --> 11T10-11T20, 6T1-6T8, 100bp ladder
Table 2. Gel box #2 arrangement
11T10 | 11T11 | 11T12 | 11T13 | 11T14 | 11T15 | 11T16 | 11T17 | 11T18 | 11T19 | 11T20 | 100bp ladder | 6T1 | 6T2 | 6T3 | 6T4 | 6T5 | 6T6 | 6T7 | 6T8 |
Gel box #3: AMBL Box: 8 wells --> 6T9-6T15, 100bp ladder
Table 3. Gel box #3 arrangement
6T9 | 6T10 | 6T11 | 6T12 | 100bp ladder | 6T13 | 6T14 | 6T15 |
- Machine Conditions: 70V, 60mAmps @ 60 min, 0.5x TBE buffer
- DNA: 10uL, loading dye:2 uL
Gel results:
- Gel box #1:
- Gel box #2:
- Gel box #3:
Vicki Ma 21:21, 1 July 2010 (EDT)
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