IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/06

From OpenWetWare
Jump to navigationJump to search
iGEM Project name 1 Main project page
Previous entry      Next entry

Monday 6th September

  • Multiple overnight restriction digests were set up as shown bellow:

1) Part BBa_E0040: 

  Plasmid - 10μL
  Pst1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

2) Part BBa_E0040: 

  Plasmid - 10μL
  Xba1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

3) pgaA promoter: 

  Plasmid - 10μL
  Spe1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

4) CsrB promoter: 

  Plasmid - 10μL
  Spe1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

5) HapR promoter: 

  Plasmid - 10μL
  Spe1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

6) Cholera System: 

  plasmid - 10μL
  Hind3 - 2μL
  Nco1 - 2μL
  Buffer 2 - 2μL
  H2O - 4μL



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:The_University_of_Sheffield/2009/Notebook/Sheffield_iGEM_2010/2010/09/06&oldid=1006733"