IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/16

iGEM Project name 1	Main project page Previous entry      Next entry
Monday 16th August: Poured more antibiotic agar plates, (Km, Amp, Km + Amp) Set up a restriction digest with pACYC, pCDF and pCOLA with EcoR1 and Pst1 enzymes: Plasmid- 10μL Pst1 - 2μL NcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL Carried out gel electrophoresis [[1]] of the digested plasmids. Made overnight starter cultures of MG1655 WT. Made new LB.