IGEM:PennState/2006/Plate-Reading

Plate Reading

Plate Reading

Time: variable

About

This is a kinetic assay for induction by HSL over a 107-magnitude concentration. Each construct is measured in triplicate.

Media/Reagents/Materials

Plates that are needed: 3+X shaker/growing, X fluorescence, where 2X is number of time points desired.

• M9+0.1%YE+antibiotic plates
• 210 mL M9+0.1%YE+antibiotic
• 96 well growing plates
• 96 well fluorescence plates
• 96 well clear bottom absorbance plates

Pre

1. Condition parts on M9+0.1%YE+antibiotic minimal media plates
2. Inoculate 3 single colonies from these plates into M9+0.1%YE+antibiotic media overnight
3. Autoclave 96 well shaker/growing plates
4. Dilute cells down to OD600=0.1

Plate Reading

1. Do not put cells on ice!

For GFP, assay at 30ºC

Main