IGEM:Peking/2007/Switch-Notebook/2007-9-10
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Colony PCR
Finally we have some colonies that may turn out right
Rescue by precipitation of all fragments
Ex-Taq PCR of RS1 and EGFP
We got the two fragments
ligation
I7100 with CIind-
I13521 with OR21/321/EGFP
Forget self-ligation!
