Tandem OriT by Qu Mingzhi
Amplification Culture of F-OriT->pSB1A2
- Culture at Amp+ plate for 12 hours.
- select Positive OriT-pSB1A2 Colonies from Plate,Culture in liquid LB,waiting for mini-prep.
- pSB1A2 self-ligation nagetive-control did not grow.
Lock & Key By Yu Tao
oriT Knock Out
- By Xu Anting
- Got sequencing-corrected strains of F and R751.
- All pSC101 sent for sequencing (7 colonies) have the same point mutations in several regions. I wonder if our strain from CGMCC is different from that in NCBI database. I will go on with these strains and meanwhile repeat the oriT-pSC101-deleted construction.
Amplification culture of FRS
- Amplification culture of sequenced strains: F (No. 3); R751 (No. 3); pSC101 (No. 4). Shake with LB-Amp+ liquid in 37℃ for 16 hours.