double digesting test of E0040->pSB3k3
- digesting (GFP+pSB3K3) and E0040 with EcoR1/PstI.
- Digestion system contains :
- (GFP+pSB3K3): 2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 5uL plasmid, 13uL ddH2O.
- E0040:2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 4uL plasmid, 13uL ddH2O.
- e0040->pSB3k3-1 @ EcoRI/Pst1
- e0040->pSB3k3-2 @ EcoRI/Pst1
- control:e0040 @ EcoRI/Pst1
- I7100 @ EcoRI/Pst1
- e0040->pSB3k3-1 plasmid
- e0040->pSB3k3-2 plasmid
- Marker: DL2000 plus
- I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.