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Aleksandra
Transformation of the TURBO cells with yesterday's ligations (150µl per transformation)
- Pmed + mRFP
- Pst + mRFP
- Pmed/strRBS/LuxR + attC/term/attC/mRFP
- Pmed/medRBS/LuxR + attC/term/attC/mRFP
- Pmed/weakRBS/LuxR + attC/term/attC/mRFP
- Pstr/weakRBS/LuxR + attC/term/attC/mRFP
- Pstr/medRBS/LuxR + attC/term/attC/mRFP
- Pstr/strRBS/LuxR + attC/term/attC/mRFP
- Ps ctrl
- Pm ctrl
- Pm/weakRBS/LuxR ctrl
- Psstr/strRBS/LuxR ctrl
Raphaël
Minipreps
Elution in 60µl water
- Pm/weakRBS/LuxR
- Ps/weakRBS/LuxR
- Pm/medRBS/LuxR
- Ps/medRBS/LuxR
- Pm/strRBS/LuxR
- Ps/strRBS/LuxR
Elution in 30µl water
- weakRBS/TetA(C)
- medRBS/TetA(C)
- strRBS/TetA(C)
- weakRBS/LacZα
- strRBS/LacZα
- 11 minipreps of attC/term/attC/mRFP
Théotime
Overnight Culture in 12mL LB AMP.
- Pm/weakRBS/LuxR/attC/term/attC/mRFP (x3)
- Ps/weakRBS/LuxR/attC/term/attC/mRFP (x2)
- Pm/medRBS/LuxR/attC/term/attC/mRFP (x1)
- Ps/medRBS/LuxR/attC/term/attC/mRFP (x2)
- Pm/stdRBS/LuxR/attC/term/attC/mRFP (x3)
- Ps/stdRBS/LuxR/attC/term/attC/mRFP (x1)
- Ps/RFP (x4)
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Memo cell
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