IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/annexe

Notebook	Design	Roadmap	Meetings and to-dos	Protocols	Bibliography	Previous Biosafety iGEM projects

Semantic containment project

Annexes

---

Sequences

Prefix 1

Before sequences starting with ATG GAATTCGCGGCCGCTTCTAG

Prefix 2

Before sequences starting with no ATG GAATTCGCGGCCGCTTCTAGAG

Suffix

TACTAGTAGCGGCCGCTGCAG

Plasmid 1 : pSB3C5::S0/1/6

• KanR : [1]

NB :

• P- & -S means Prefix and Suffix added

Geneious file : File:Semantic.geneious (right click, save under...)

P-KanR all serines changed-S

 GAATTCGCGGCCGCTTCTAGATGTAGCATATTCAACGGGAAACGTAGTGC
 TAGAGGCCGCGATTAAATTAGAACATGGATGCTGATTTATATGGGTATAA
 ATGGGCTCGCGATAATGTCGGGCAATAGGGTGCGACAATCTATCGATTGT
 ATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTTAG
 GTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGA
 ATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATG
 CATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTA
 GAAGAATATCCTGATTAGGGTGAAAATATTGTTGATGCGCTGGCAGTGTT
 CCTGCGCCGGTTGCATTAGATTCCTGTTTGTAATTGTCCTTTTAACTAGG
 ATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTG
 GTTGATGCGTAGGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACA
 AGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTAGCCGGATTAGGTCG
 TCACTCATGGTGATTTCTAGCTTGATAACCTTATTTTTGACGAGGGGAAA
 TTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCA
 GGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTAGCCTTAGTTAC
 AGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAA
 TTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCC
 GCTGCAG

Fragment 1

 GAATTCGCGGCCGCTTCTAGATGTAGCATATTCAACGGGAAACGTAGTGC
 TAGAGGCCGCGATTAAATTAGAACATGGATGCTGATTTATATGGGTATAA
 ATGGGCTCGCGATAATGTCGGGCAATAGGGTGCGACAATCTATCGATTGT
 ATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTTAG
 GTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGA
 ATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATG
 CATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTA
 GAAGAATATCCTGATTAGGGTGAAAATATTGTTGATGCGCTGGCAGTGTT
 CCTGCGCCGGTTGCATTAGATTCCTGTTTGTAATTGTCCTTTTAACTAGG
 ATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACG

Fragment 2

 GATTAGGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTT
 GCATTAGATTCCTGTTTGTAATTGTCCTTTTAACTAGGATCGCGTATTTC
 GTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTGGTTGATGCGTAG
 GATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGA
 AATGCATAAGCTTTTGCCATTCTAGCCGGATTAGGTCGTCACTCATGGTG
 ATTTCTAGCTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGT
 ATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCAT
 CCTATGGAACTGCCTCGGTGAGTTTTAGCCTTAGTTACAGAAACGGCTTT
 TTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCAT
 TTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCCGCTGCAG

Plasmid 2 : pSB1A3::supD_T1

BBa_K228001 - SupD-tRNA

 CAATTCGGAGAGATGCCGGAGCGGCTGAACGGACCGGTCTCTAAAACCGG
 AGTAGGGGCAACTCTACCGGGGGTTCAAATCCCCCTCTCTCCGCCACTAC
 AGATCCTTAGCGAAAGCTAAGGATTTTTTTTAAGCT

BBa_B1006 - Terminator 1

AAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTT