IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/23/Shan Shen
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0:30
Digest SupD+1-23L 1, 2, 3 to test whether the clones are correct.
| Total | 20μL |
| Plasmids | 8μL |
| Xba1 | 1μL |
| Pst1 | 1μL |
| Buffer | 2μL |
| ddH2O | 8μL |
PCR to test at the same time.
| Total | 10μL |
| Template | Clones |
| dNTP | 2μL |
| Buffer | 1μL |
| Taq | 0.5μL |
| For-primer | 0.5μL |
| Rev-primer | 0.5μL |
| ddH2O | 5.5μL |
1:20
Start to digest.
1:45
Start to PCR.
10:40
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, colony 1 digestion products, colony 2 digestion products, colony 3 digestion products, positive control, PCR products of colony 1, PCR products of colony 2, PCR products of colony 3.
Results:
This result indicates that all the three colonies are correct.
