IGEM:MIT/2007/Notebook/2007-8-4

Double Digests!!!

5.3 µl     I14032 ( 188.4 ng/µl)
0.5 µl     BSA
5 µl       NEB2 Buffer
1 µl       SpeI
1 µl       PstI
37.7 µl      H20
--------------------------
50 µl      Total

42.5 µl    R0051 (16.8 ng/µl)
0.5 µl      BSA
5 µl         NEB2 Buffer
1 µl         SpeI
1 µl         PstI
--------------------------
50 µl       Total

12 µl       B0034 (84.9 ng/µl)
0.5 µl      BSA
5 µl         NEB3 Buffer
1 µl         XbaI
1 µl         PstI
30.5 µl    H2O
--------------------------
50 µl       Total

5 µl          CPX (1µg/5µl)
0.5 µl       BSA
5 µl          EcoRI Buffer
1 µl          EcoRI
1 µl          SpeI
37.5 µl     H2O
--------------------------
50 µl        Total

16  µl      B0014 (62.8 ng/µl)
0.5 µl      BSA
5 µl         NEB2 Buffer
1 µl         XbaI
1 µl         EcoRI
26.5 µl    H2O
--------------------------
50 µl       Total