IGEM:MIT/2007/Notebook/2007-7-8

From OpenWetWare

Jump to: navigation, search

Miniprep of F2620, B0034, E1010 From 7/5/07 Plating

Part(Plate #/ Pick#/ Nanodrop)

B0034 #1
   Plate 2/ Part 2/ 84.4 ng/µl
B0034 #2
*   3/ 1/ 84.9 ng/µl
F2620 #1
*   3/ 2/ 134.5 ng/µl
F2620 #2
*   2/ 2/ 113.6 ng/µl
E1010 #1
*   1/ 2/ 162.8 ng/µl
E1010 #2
*   2/ 1/ 121.5 ng/µl

Digestion of F2620, B0034, pSB3k3

===F2620===
8.8 µl    F2620
0.5 µl    BSA
5 µl      NEB2 Buffer
2 µl      EcoRI
2 µl      SpeI
31.7 µl   H20

===B0034===
11.8 µl   B0034
0.5 µl    BSA
5 µl      NEB3 Buffer
2 µl      XbaI
2 µl      PstI
28.7 µl   H20

===pSB3k3===
38.2 µl   pSB3k3
0.5 µl    BSA
5 µl      NEB2 Buffer
2 µl      EcoRI
2 µl      SpeI
2.3 µl    H20
  • Add Enzymes Last
Personal tools