IGEM:MIT/2007/Notebook/2007-7-13
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Checked Plates of Transformed 3A XL-1
- LB+Kan Plate - all had significant growth
- LB+Kan+Amp:
- 1: very small colonies
- 2: no growth
- 3: some bits of dead cells on a streak, otherwise no growth
- 4: many colonies
- 5: no growth
- 6: 9 colonies
- 7: no growth
- 8: no growth
- The growth of colonies on #4 supports the theory that those cells received both pSB3k3 with F2620 and B0034 (Kan, medium copy) and religated pSB1A3 with just F2620 (Amp, high copy)
Diluted LC#1,2 of XL-1 3A in LB+Kan
- Since those only grew on LB+Kan and not LB+Kan+Amp plate
- for miniprep tomorrow
- 37C at 3:30pm
Transformed Standard Assembly Ligation of E1010 into pSB3k3 from Yesterday
- made 8 plates total, 2 samples
- 37C @ 4:30pm
Received from Tom Knight digested pSB(1A3, 1AT3, 1AK3) vectors & Chemically Competent Cells
- vectors digested with EcoRI and PstI, placed in -20C
- cells placed in -80C, labeled iGEM2007
Meeting with Drew and TK Notes
- check to see if Kan resistant already
- ask for digested vector--tom knight is on it
- terminators
- make a color scheme as orgzn tool to display
- Full product cycle
- What to do at the end?
- Hidden costs for disposal
- Target market
- rich
- poor too
- Find Mass PCR things
- Different application
- modification; disrupt bioaccumulation in food chain
- make a salt or something, mercury crystal ->more valuable, recoverable, disposal
- Diane Newman 3rd floor.
- Magnetites, etc., go talk to her
- Safety conversations
- EHS website for Mercury
- MerA leave it in?
- Could be MerB/C
- Price of Mercury/Cadmium
- List of Target Metals
- Then explain why this one, and not that ones
- Bad/good/metal-specific opportunities
- any number of good reasons/Markets/etc
- Compare different ones
- Does Mercury contamination exist in medium sized?
- Other bacterial species
- Pseudomonus--robust
- So measurements/experiments
- Plan of attack
- Figure out how to quantitate, figure out parameters about stickiness
- Measurement of ions
- Figure out which version of mercury, +1? Most have been +2
- Presentation Divisions
- Put together part of the talk
- Do earlier than later
- Make our own slides