IGEM:MIT/2006/Notebook/2006-9-12

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to do

  1. plate reader: 7 tubes in 37 room (amp, a/t, a/k backbone controls) done: dilutions (SP), loading at 5pm (KB)
    • E 1-3 = J450987 a/k
    • E 4-6 = R0040-E0840 amp
    • E 7-9 = J45270 a/t
    • E 10-12 = osmY-E0840 SN a/t
    • F 1-3 = J45017 amp
    • F 4-6 = osmY-Q-E0840 FN a/k
    • 7-9 = osmY-E0840 FN amp
  2. colony PCR lots of colonies from the y0078 and y0080 plates: looking for an ATF1 length insert!! done: pcr and LCs (SP) 2 gels (KB)
    • -- ALL FAILED!!!! boo
  3. sequence analysis done: KB
    • 320s are definately correct
    • all 399s incorrect
    • 397 definately correct for final BB box
    • 1148-mutated J45220 definately correct/successful mutagenesis
  4. NOTE: unfortunately, i don't think that the 6 sample sequence order of the 398-muts was ever completed today. since it is way past 5, i moved this task to tomorrow's wiki (KB)
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