IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/05

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  • Transformed I13600 and I13602 from Kit.
  • Ligated 001(1) +I13507 ; 001(3) + I13507; 001(4) + I13507; 001(5) + I13507; 001(10) + I13507. ---> E set of Ligations.
  • Inoculated the following:
  • 502(B); 500(B); 019(C) ---> Chl parts.
  • Inoculated I13600; I13602; I13521; J04450; J+S(B); 019(E)-----> Amp





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