IGEM:IMPERIAL/2009/M3/Assays

Assays & Protocols

1) Promoter Characterisation

Promoter characterisation
F2620 characterisation

2) Thermoinduction

GFP test of cI + p(lambda)
Effect of Temperature
Beta Galactosidase (back up in case GFP does not work)

3) Killing strategy

Colony Forming Units

Membrane Staining ( This may move to module 2)

Effect of Temperature on killing rate

Restriction Enzyme Assay

Methylation Assay