IGEM:IMPERIAL/2008/Prototype/Wetlab/Shopping List
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Materials | Quantity | Company | Order No. | Cost | Date |
Glycerol | |||||
Glucose | |||||
MgSO4.7H2O | 500g | Sigma | M1880 | ||
Casamino Acids | |||||
Yeast Extract | |||||
CaCl2 | 500g | Sigma | C1016 | ||
(NH4)2SO4 | 500g | Sigma | A4418 | ||
KH2PO4 | |||||
Sodium Citrate | |||||
EGTA | 10g | Sigma | E3889 | ||
EDTA | |||||
Trypton | |||||
Minimal Salts | 250g | Sigma | M6030 | ||
NaCl | 2.5kg | Fisher | BP358 | ||
Ammonium Citrate | 100g | Sigma | F5879 | ||
Casein hydrolysate | |||||
PEG 6000 | 250g | Sigma | 81255 | ||
KCL | 1kg | Fisher | BP366 | ||
MgCl | 100g | Sigma | M8266 | ||
Midi kit | |||||
Mini Kit | |||||
Gel Extraction | |||||
Ampicilin | 25g | Sigma | A9518 | ||
Kanamycin | 25g | Sigma | K4000 | ||
Tetracyclin | 5g | Sigma | T7660 | ||
PCR clean up kit | |||||
Pfu ultra fusion | |||||
PBS x 50 | |||||
Agar | |||||
Agarose | |||||
Polyacrylamide | |||||
Tris | 1kg | Fisher | BP152 | ||
Ammonium persulfate (APS) | 25g | Fisher | BP179 | ||
SDS | 1 litre 20% | Fisger | BP1311 | ||
TEMED | 25ml | Fisher Scientific | T/p190/04 | ||
TAEx50 | |||||
Protein Marker | |||||
DNA Marker | |||||
Ethanol | |||||
Acetic Acid | |||||
Commasie Brilliant Blue R | |||||
β Mercapto Ethanol | |||||
DTT | |||||
Bromophenol Blue | |||||
Grams Iodine Solution | 8FOZ | Sigma | HT902 | ||
IPTG | |||||
HEPES | 1kg | Fisher | BP310 | ||
Glycine | 1kg | Fisher | BP318 |