IGEM:IMPERIAL/2008/New/Protocols/Constitutive
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Equipment
Reagents and Materials
ProtocolDay 1
Erika says: Work out how many cultures you are going to need (nine by the looks of things below!) and do the above nine times... J-We can start with just one overnight culture then make inncolulate 3 x fresh media in the morning. The reason for 9 is that for each of the cultures we make on day 2, we pipette 3 aliquots from it. This helps us to average for any pipetting errors when the plate is loaded, whereas the different cultures help to give independent repeats for the experiements. Day 2
Erika says: Can we not innoculate and put straight into the plate reader? Why do we have to wait until exponential phase? J-Yeah we can put it in straight away and see what happens. My only concern is that I do not know how happy the bacteria will grow in the plate. The population is small but the available media is limited. Clearly this needs to be determined. Can the plate reader measure OD? That would be handy. -Yes we measure the O.D.600 |