IGEM:IMPERIAL/2007/Projects/Hrp System/Systems/Hrp Device 2/TestingValidation
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| Super Parts | links to higher level parts if applicable | |
|---|---|---|
| Actual Part |  | |
| Sub Parts | RBS (Elowitz) BBa_B0034 | Double terminator (B0010-B0012) BBa_B0015 |
Equipment
- Twinkle Plate Reader Fluorometer
- 96 well plate
- Eppendorf Tubes
- Gilson Pippettes
- Plastic Curvetts
- Incubator 37o shaker
- Vortex and Centrifuge
- Spectrometer
Materials and Reagents
- M9 minimal media 40μg/ml Ampicillin
- Inducer of promoter at varying concentrations
- Recombinant acGFP of known concentration
- Agar Plates
- E.coli strain containing vector
Protocol
Device 2 Protocol Two promoters are tested each have a range of 8 inducer concentrations.
- pBad will have a range of 0 to 100mM added at intervals of 12.5mM Protocol
- pLac will have a range of 0 to 0.1mM IPTG added at intervals of 0.0125mM. Protocol
Device Test Construct
For this test construct we initially need to run preliminary tests in order to find out what time intervals and what input ranges we need. To run this the range of concentrations used for promoters will be used. This device uses a pLac promoter, the proposed range of concentrations is going to be defined from the promoter experiment, this is use a range of concentrations to cover an appropriate range of promoter. From looking at literature an appropriate range is 0-100μM.
