- We need a standardized units to compare expression in different chassis.
- Ideal unit for this is PoPS (Polymerase per Second), however this cannot directly be measured. Currently the best approach is to measure indirectly using Rate of GFP molecules sysnthesised sec-1 CFU-1 (CFU meaning Colony forming units which is a measure of cells)
- We would like to use this approach, however, there is no CFU in in-vitro and in-veso. We need to change CFU so we can relate it to in-vitro and in-veso.
List what we plan to characterize that are chassis dependent characteristics e.g. temp, life span of chassis
Construct Dependent Characteristics
List what we plan to characterize that are construct dependent characteristics e.g. change of expression.