IGEM:IMPERIAL/2006/project/Oscillator/Modelling/Realistic Values

Known

• Gene expresion: 2 proteins/sec/plasmid
  • Source: Modelling the Lux/AiiA Relaxation Oscillator

• LuxR Half-Life: 60min
  • Can be varied between 40 and 100min using furones
  • Source: Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover

• AHL Half-Life: 3 Hours (Conflicting information)
  • Source: Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus
  • Conflict: Cell-Cell Communication by Quorum Sensing sugests AHL half-life is 24 hours

• aiiA Half-Life: 24hrs
  • Could be halved by degredation tagging
  • Source: Dr Mann

• LuxI Vmax: 1.1mol HSL/mol LuxI/min
  • Can be increased by over a factor of at least 1000 with addition of SAM and hexanoyl-ACP
  • Source: Acyl-homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein
  • Source: Cell-Cell Communication by Quorum Sensing

• LuxI Km:
  • 1. For hexanoyl-ACP: 9.6uM
  • 2. For SAM: 130uM
  • Source: Acyl-homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein

• aiiA Vmax: 68.6 nmol/min per mg = 1.715mol/min per mol
  • Source: The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase

• aiiA Km: 0.74mM
  • Source: The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase

• E.coli Volume: 0.88x10^-15 L
  • Source: Institute for Biomolecular Design

Guessed

• LuxI Half-Life: 60min
  • Reason: Similar to LuxR Half-Life

Needs to be researched

• Assocaition constant between LuxR and HSL

• Dissocaition constant between LuxR and HSL

• How well does LuxR activate Genes?