TO DO: *Miniprep of J37024 and J37031 (from the o/n ligations) *Ligations and Electroporations: J37022 (AiiA test construct) J37025 (to be used to construct the final poly/monocistronic predator parts) J37018 (to be used to construct the final monocistronic predator part) J37020 (the polycistronic prey test construct)
- J37024 colonies 2 and 3 successful (colony 1 unsuccessful)
- J37031 all three colonies successful
Ligations and Electroporations
- Ligation set up today.
- Dr. Mann said he'd transform if he was still around later today or else he'll do it tomorrow...things are looking up, we may get our test construct yet.
- Using new blunt-end kit, the ligation of the Cre-Lox part into a blunt-ended plasmid
- Left to ligate overnight