Strains, Plasmids, and Trasformation
1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.
2. Galvin  Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
3. Antunes, et. al.  A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity
4. Everything you want to know about Arabidopsis 
- Book: Agrobacterium Protocols
- Chuang CF and Meyerowitz EM. . pmid:10781109.
Markers and Genetic Fences
Dual selection system ideas
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
- this paper has some parts that could be useful as kill-switches
- A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.
- steroid-inducible chlorophyll knockdown
Peanut and Tomatoes review
- Singh MB and Bhalla PL. . pmid:18467156.
Peanut profilins: Ara h knock-down
- Dodo H, Konan K, and Viquez O. . pmid:15659267.
Tomato profilins: Lyc e knock-down
- Design of tomato fruits with reduced allergenicity by dsRNAi-mediated inhibition of ns-LTP (Lyc e 3) expression. Le LQ, Lorenz Y, Scheurer S, Fötisch K, Enrique E, Bartra J, Biemelt S, Vieths S, Sonnewald U. Plant Biotechnol J. 2006 Mar;4(2):231-42. pmid:17177799
- Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. Le LQ, Mahler V, Lorenz Y, Scheurer S, Biemelt S, Vieths S, Sonnewald U. J Allergy Clin Immunol. 2006 Nov;118(5):1176-83. Epub 2006 Sep 8. pmid:17088146
Orange profilin: Cit s 2 (characterized, but not knocked down yet)
- Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. López-Torrejón G, Ibáñez MD, Ahrazem O, Sánchez-Monge R, Sastre J, Lombardero M, Barber D, Salcedo G. Allergy. 2005 Nov;60(11):1424-9. pmid:16197476
-  Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde
- (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid, MIT iGEM 2006
- Sinha AK, Sharma UK, and Sharma N. . pmid:17886091.
De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker's yeast (Saccharomyces cerevisiae). http://www.ncbi.nlm.nih.gov.ezp-prod1.hul.harvard.edu/pubmed/19286778
- Theerasilp S and Kurihara Y. . pmid:3403544.
- Stewart C Jr, Kang BC, Liu K, Mazourek M, Moore SL, Yoo EY, Kim BD, Paran I, and Jahn MM. . pmid:15918882.
- TRPV1, the only known receptor (a transient receptor potential channel) for capsaicin.
- Piperine, the active piquant chemical in black pepper
- Allyl isothiocyanate, the active piquant chemical in mustard, radishes, horseradish, and wasabi
- Allicin, the active piquant flavor chemical in uncooked garlic and onions (see those articles for discussion of other chemicals in them relating to pungency, and eye irritation)
- Naga Jolokia pepper, the world's most capsaicin-rich fruit
- Discovery and development of TRPV1 antagonists
- Capsinoids are similar in structure to capsaicin, but lack the extreme pungency.
- Raksakulthai R and Haard NF. . pmid:12940418.
- Ashihara H, Sano H, and Crozier A. . pmid:18068204.
can we eat it for the iGEM project (human testing)? Can we hand it out to be eaten (FDA)? Patent/copyright issues? Can we have actual tranformed plants ready by the jamboree