IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week8/Widgetry

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Contents

Goals for this week

  1. Run co-culture test with DH5α, DH5α/pUC19, and Shewie
  2. Move chambers to the "dark" to prevent a reaction from happening with light and oxygen
  3. Test light-repressible system on plates

Monday: August 11, 2008

  • cleaned chambers
  • moved setup to a cabinet to have system in the "dark"

DH5α, DH5α/pUC19, and Shewie co-culture experiment setup

  • Chamber 1: DH5α + wt Shewie + Lactose
  • Chamber 2: DH5α + wt Shewie + Lactate (pos. control)
  • Chamber 3: DH5α + wt Shewie (neg. control)
  • Chamber 4: DH5α/pUC19 + wt Shewie + Lactose
  • Chamber 5: DH5α/pUC19 + wt Shewie + Lactate (pos. control)
  • Chamber 6: DH5α/pUC19 + wt Shewie (neg. control)
  • Chamber 7: wt Shewie + Lactose (neg. control)
  • Chamber 8: DH5α + Lactose (neg. control)
  • Chamber 9: DH5α/pUC19 + Lactose (neg. control)

Tuesday: August 12, 2008

Wednesday: August 13, 2008

RU1012 Light Repressible system experiment

  • Streaked plates.
  • All Cm/Amp plates
Dark
  • Plate 1: RU1012 w/ plasmids
  • Plate 2: RU1012
  • Plate 3: Negative control (No bugs)
Light
  • Plate 1: RU1012 w/ plasmids
  • Plate 2: RU1012
  • Plate 3: Negative control (No bugs)

Thursday: August 14, 2008

RU1012 Light Repressible system experiment

  • Spread plates to try to create lawn.
  • All Cm/Amp plates
Dark
  • Plate 1: RU1012 w/ plasmids
  • Plate 2: RU1012
  • Plate 3: Negative control (No bugs)
Light
  • Plate 1: RU1012 w/ plasmids
  • Plate 2: RU1012
  • Plate 3: Negative control (No bugs)

Friday: August 15, 2008

RU1012 Light Repressible system experiment

  • Spread plates to try to create lawn.
  • All Cm/Amp plates
Dark
  • Plate 1: RU1012 w/ plasmids
  • Plate 2: RU1012
  • Plate 3: Negative control (No bugs)
Light
  • Plate 1: RU1012 w/ plasmids
  • Plate 2: RU1012
  • Plate 3: Negative control (No bugs)
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