IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week1/Light

Transformations of P11, P15-20

Colony counts

6/23:

Name	Description	Origin	Size	Marker	Number of Colonies
P11	Light responsive system, dual regulation	pUC19-derived pMB1	4333	Amp/Cm	224
P15	GFP with Tet promoter	ColE1	937	Amp	360
P16	Tet repressible promoter	pMB1	54	Amp	264
P17	Inverter (TetR w/o promoter and Tet promoter)	OriS, P1 lytic, F1	902	Kan	2
P18	lambda promoter (cI regulated)	pMB1	49	Amp	112
P19	RFP with lambda promoter	pMB1	918	Amp	104
P20	GFP (LVA tagged) with Lac promoter	pMB1	1122	Amp, Kan	136

Restreaking

6/23: P16, which has GFP under a constitutive Tet promoter did not appear to fluoresce (none of the fluorescent constructs did), so we restreaked all the of the plates (with the same antibiotic on which they were originally grown). They were left in the 37 degree incubator along with a blank Kan and a blank Amp plate (- controls).

Overnight cultures

6/23: The above was repeated in 5mL LB liquid cultures instead of plates.