IGEM:Harvard/2006/vlau/Notebook/2006-7-26
From OpenWetWare
Jump to navigationJump to search
PEG precipitation
1. Rxn Mixtures
1: 20 μL c4.0Fb + 20 μL 20%PEG + 160 μL dH2O 2: 20 μL c4.0Fb + 30 μL 20%PEG + 150 μL dH2O 3: 20 μL c4.0Fb + 40 μL 20%PEG + 140 μL dH2O
2. Protocol
- incubated on ice for 15 min. - spun @ 16,000 rpm for 10 min. - supernatant removed into separate tubes and pellet resuspended in 1x volume folding buffer to supernatant