IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-14
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CIP treatment (fail)
CIP treated the '0S' sequence, which is J36010/SP. Will need to re-CIP treat.
Gel extraction
- 1: Ladder
- 2: undigested 36010
- 3: digested 36010 /XP marker
- 4: digested 36010 /XP
- 5: digested 36010 /XP
- 6: digested 36010 /XP (not shown)
- 7: digested 36010 /XP (not shown)
- 8: digested 36010 /XP marker
- 9: Ladder
- 10: digested 36011 /SP
- 11: digested 36011 /SP (not shown)
- 12: Ladder
- 13: digested 36010 /SP marker
- 14: digested 36010 /SP
- 15: digested 36010 /SP
- 16: digested 36010 /SP
- 17: digested 36010 /SP
- 18: digested 36010 /SP marker
- 19: undigested 36010
- 20: Ladder
The gel ran as if there was a lot of genomic DNA; additionally there is no KaiC /XP expected 1.8kb insert in lanes 3-5; and notice in lane 10 that the supposed KaiB /SP is a smudge.
Will poststain next gel to verify results.
Gel#2:
- 1: ladder
- 2: undigested 36010
- 3: undigested 36011
- 4: digested 36011 /sp marker
- 5: digested 36011 /sp (not shown)
- 6: digested 36011 /sp (not shown)
- 7 digested 36011 /sp marker
- 8: ladder
Innoculation
Reinnoculated M27 and B18.