IGEM:Groningen/Notebook/iGEM 2011/2011/06/10

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10-06-11 Colony PCR of transformants! Taq 10× buffer: 40μl dNTPs 10mM: 8μl Forward Biobrick primer: 8μl Reverse Biobrick primer: 8μl Taq DNA polymerase: 2μl MQ water: 334μl PCR conditions: Preheated lid: 111°C Denaturation: 94°C for 10 min. Cycle 33×: denaturation: 94°C for 30s. annealing: 60°C for 30s. Extension: 72°C for 2,5 min. Final extension: 72°C for 10 min. Store infinite at 4°C Analyse on a 1% TBE agarose gel. Asked info in team about why some PCR's fail: next time use for taq buffer not the buffer with KCI, but NH4SO4